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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (1) BJMBR (1) Autor Barreto Filho,J.B. (2) Golgher,R.R. (2) Carvalho,P.H.A. (1) Eiras,P.R.S. (1) Lopes,E. (1) Marques Júnior,A.P. (1) Rossi,R.O.D.S. (1) Mais... Palavra-chave Amino acid (1) Bovine alpha interferon (1) Cattle (1) Epitope (1) Interferon (1) Neutralization (1) Placenta (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2007 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Antiviral activity of bovine uterus and placenta induced by Newcastle disease virus Arq. Bras. Med. Vet. Zootec. Barreto Filho,J.B.; Marques Júnior,A.P.; Golgher,R.R.; Lopes,E.; Carvalho,P.H.A.; Rossi,R.O.D.S.. The antiviral activity profile of the uterus and fetal membranes from bovine placenta, induced by the Newcastle disease virus (NDV) throughout gestation, was investigated. Explants of the endometrium and caruncles were collected from the uterus, and amniochorion, allantochorion and cotyledons, from fetal placenta. Tissue cultures were induced with ~6.0 hemagglutinating units (HU) of NDV. Supernatants were concentrated 20 fold, filtered in 100kDa cut-off membranes and antiviral activity was titrated in MDBK x VSV system. Tissues of the uterus did not exhibit antiviral activity, while allantochorion and amniochorion produced antiviral factors throughout gestation. Antiviral factors were not related with IFN-alpha, gamma, tau or TNF-alpha. The antiviral... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Interferon; Placenta. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300006 N-terminal amino acids of bovine alpha interferons are relevant for the neutralization of their antiviral activity BJMBR Barreto Filho,J.B.; Eiras,P.R.S.; Golgher,R.R.. The structure-function relationship of interferons (IFNs) has been studied by epitope mapping. Epitopes of bovine IFNs, however, are practically unknown, despite their importance in virus infections and in the maternal recognition of pregnancy. It has been shown that recombinant bovine (rBo)IFN-alphaC and rBoIFN-alpha1 differ only in 12 amino acids and that the F12 monoclonal antibody (mAb) binds to a linear sequence of residues 10 to 34. We show here that the antiviral activities of these two IFNs were neutralized by the F12 mAb to different extents using two tests. In residual activity tests the antiviral activity dropped by more than 99% with rBoIFN-alphaC and by 84% with rBoIFN-alpha1. In checkerboard antibody titrations, the F12 mAb titer was 12,000... Tipo: Info:eu-repo/semantics/other Palavras-chave: Bovine alpha interferon; Neutralization; Amino acid; Epitope. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500015 Registros recuperados: 2 Primeira ... 1 ... Última

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